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POD reacting with hydrogen peroxide (H2O2) catalyzes oxidative condensation of tyramide to create very reactive intermediates, tyramide radicals. The activated tyramide covalently binds to electron-rich moieties such as tyrosine, phenylalanine and tryptophan at or close to the POD. The brief half-lifetime of the tyramide radicals restricts the depo

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